Composite

Part:BBa_K3308002:Design

Designed by: Jemy Vargehse   Group: iGEM19_Pittsburgh   (2019-10-05)


[IMPDH-1 C (1-35)]-[Tvo VMA C48 N]


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 589
    Illegal BsaI.rc site found at 74


Design Notes

Design of this part was based on predicted best site to splice. The splice site was chosen according to biochemical requirements for interactions between either ends of intein.


Source

The source of the intein sequence and characterization data is InBase

References